Gel Electrophoresis Buffers and Diluents
Vector Laboratories VECTOR VIP Peroxidase (HRP) Substrate Kit 300 ml
The Vector VIP Peroxidase (HRP) Substrate Kit produces intense violet (purple) reaction products and can be used as an alternative to DAB, or as a second color for multiple antigen labeling. Vector VIP chromogens also provides an excellent color contrast in pigmented tissues such as melanoma or retina. Vector VIP substrate can be used for both manual and automated staining methods. Stained sections should be dehydrated, cleared, and permanently mounted. The sensitivity of Vector VIP substrate is equivalent to Vector DAB Substrate.Vector VIP Substrate Kit reagents are provided in convenient dropper bottles, enough to prepare 300 ml of working solution. This product can also be used for blot-based protein detection.
Sigma Aldrich Fine Chemicals Biosciences N27 A Rat Dopaminergic Neu
Parkinsons disease is characterized by the death of dopaminergic neurons in the substantia nigra of the brain . In vitro models are critical for understanding the molecular pathology of Parkinsons disease. The N27 immortalized rat dopaminergic cell line has been widely utilized in Parkinsons research for over 20 years. However long-term passaging has led the N27 cell line to become a mixture of cell types with widely variable expression of dopaminergic neural markers such as tyrosine hydroxylase (TH).
Bioworld Laemmli SDS-Sample Buffer (6X), Reducing, 50 mL
Laemmli (SDS-Sample) 6X Buffer, Reducing An electrophoretic dye for denaturation of proteins and monitoring the front of running gel. Technical Information:Ultra Pure GradeContents of a 1X Solution: 1.5% SDS and 10% glycerol, 62.5mM Tris -HCl (pH 6.8), 0.0025% Bromophenol blue, 2% β-mercaptoethanol.DNase: None detectedRNase: None detectedProteases: None detectedStability (-20°C) >1 Year
New England Biolabs, Inc. DNase I Reaction Buffer – 6 ml
New England Biolabs supplies a 10X reaction buffer with all of its enzymes. At a 1X concentration this reaction buffer assures optimal activity of the enzyme.
![Research Products International Corp STE Buffer 1X Solution [Sodium Chloride-TRIS-EDTA], 500 Milliliters](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-S24200-500.0.JPG-250.jpg)
Research Products International Corp STE Buffer 1X Solution [Sodium Chloride-TRIS-EDTA], 500 Milliliters
Formulation:
TRIS/TRIS HCI: 10 mM
pH 8.0
EDTA: 1 mM
Research Products International Corp Laemmli Buffer Reducing, 4X Solution, 25 Milliliters
4X Formulation:
Glycerol: 40.0%
β-mercaptoethanol: 8.0%
Bromophenol Blue: 0.02%
Tris-HCl: 250 mM
Electron Microscopy Sciences EMS PHEM Buffer 500 ML
PHEM buffer has been used primarily for tissues and cell cultures being processed for immunocytochemical studies. Most antigens, especially intra cellular ones, stain better using PHEM than those processed using PBS, it also has a more limited effect on biochemical reactions and enzymes. Very good preservation of cellular ultrastructure, notably microtubules, is also an advantage of PHEM use.
Biotium DNA Gel Stains Related Products DNA Gel Stains and Accessories DNA Ladders Chemical Reagents 1/EA
1 kb DNA Ladder has 13 linear double-stranded DNA fragments from 250 bp to 10 kb The 1 kb and 3 kb bands contain more DNA to provide internal orientation Includes one vial of DNA ladder 100 ng/uL in TE Buffer (500 uL) Note Does not include 6X DNA loading buffer (available separately)
Biotium DNA Gel Stains Related Products DNA Gel Stains and Accessories DNA Ladders Chemical Reagents 1/EA
100 bp DNA Ladder in TE Buffer has 11 linear double-stranded DNA fragments from 100 bp to 1500 bp The 500 bp band contains more DNA to provide internal orientation Includes one vial of DNA ladder 100 ng/uL in TE Buffer (500 uL) Note Does not include 6X DNA loading buffer (available separately)
Electron Microscopy Sciences R-Universal Epitope Recovery Buffer (10x stock) 125 ML
EMS UNIVERSAL Epitope Recovery Buffer is a novel (patent-pending) technology for epitope recovery, primarily based on reversing the fixation effects of formaldehyde, which created links primarily between ε-amino groups of lysine and other amino-groups. In extensive testing the buffer has shown excellent results when used with different antibodies, including those that require low or high pH buffers or protolithic treatment for successful staining.
The EMS Universal Buffer may be used in any heat-induced epitope recovery system (the time and temperature of treatment should be tested), but was specifically adjusted for tissue sections processing in our Antigen Retriever (62700 Series). Using our Universal Buffer in our Retriever guarantees the highest rate of success in recovering epitope for any antibody, especially one that was never previously used on formalin-fixed sections. Shelf Life: 12 months.
Surmodics Inc 450 nm Stop Reagent for TMB Microwell Substrates, 100mL
450 nm Stop Reagent for TMB Microwell Substrates (100 mL) is a proprietary dry blend used to stop TMB microwell substrate reactions causing a color change from blue to yellow. The yellow color is read in at 450 nm.